RiboMAX Large Scale RNA Production System-T7 from Promega

Product Specs
ItemRiboMAX Large Scale RNA Production System-T7
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1300
Quantity1 system

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Using sno-lncRNAs as potential markers for Prader-Willi syndrome diagnosisRNA BiologyDecember 31, 2023Jiu-Ru Sun, Liang-Zhong Yang, Yang-Li Dai, Huang Wu, Siqi Li, Yi-Feng Xu, Youkui Huang, Hao Wu, Zheng Shen, Chaochun Zou, et al.RNA for gRNAs were synthesized by in vitro transcription with T7 RNA polymerase (Promega, Cat. No. P1300) according to the manufacturer’s protocol. The crRNA and target RNA were then purified by gel electrophoresis

(2) Epitranscriptome analysis of NAD-capped RNA by spike-in-based normalization and prediction of chronological ageiScienceDecember 15, 2023Dean Li, Shuwen Ge, Yandong Liu, Miaomiao Pan, Xueting Wang, Guojing Han, Sili Zou, Rui Liu, Kongyan Niu, Chao Zhao, et al.in vitro transcription, 10 μM of double-stranded DNA (dsDNA) template in 100 μL transcription buffer (Promega, catalog: P1300), along with 1 mM of each of GTP, CTP and UTP, with 4 mM NAD (for NAD-RNA) or 4 mM m7GpppA (New England Biolabs

(3) A single amino acid substitution in the capsid protein of Zika virus contributes to a neurovirulent phenotypeNature CommunicationsOctober 26, 2023Guang-Yuan Song, Xing-Yao Huang, Meng-Jiao He, Hang-Yu Zhou, Rui-Ting Li, Ying Tian, Yan Wang, Meng-Li Cheng, Xiang Chen, Rong-Rong Zhang, et al.In vitro transcribed viral RNA was prepared using Ribomax T7 large-scale RNA production kit (Promega, P1300) and purified using Purelink RNA mini kit (Thermo Fisher Scientific, 12183018 A). The RNA was then transfected

(4) Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456Signal Transduction and Targeted TherapyOctober 19, 2023Jingjing Wan, Zhen Zhang, Chennan Wu, Saisai Tian, Yibei Zang, Ge Jin, Qingyan Sun, Pin Wang, Xin Luan, Yili Yang, et al.in vitro transcription using the T7 RNA transcription system (Large Scale RNA ProductionSystem-T7, Promega, cat. no. P1300) and biotin-labeled with the Pierce™ RNA 3’ End Desthiobiotinylation Kit (Thermo Scientific, cat. no.

(5) Recognition of polymorphic Csd proteins determines sex in the honeybeeScience AdvancesOctober 6, 2023Marianne Otte, Oksana Netschitailo, Stefanie Weidtkamp-Peters, Claus A. M. Seidel, Martin Beyecommercial assays,Critical commercial assays,Critical commercial assays RiboMAX Kit,Promega,Catalog no. P1300 MEGAclear Kit,Thermo Fisher Scientific (Ambion),Catalog no. AM1908 RevertAid First Strand cDNA Synthesis Kit

(6) A CRISPR-based rapid DNA repositioning strategy and the early intranuclear life of HSV-1eLifeSeptember 13, 2023Juan Xiang, Chaoyang Fan, Hongchang Dong, Yilei Ma, Pei XuIn vitro sgRNA transcription was performed using the Ribomax T7 large-scale RNA production kit (Promega, #P1300) according to the manufacturer’s instructions. In short, 2–4 μg of template DNA containing a T7 promoter

(7) DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in ArabidopsisSci AdvAugust 11, 2023Jincong Zhou, Xue Lei, Sarfraz Shafiq, Weifeng Zhang, Qin Li, Kuan Li, Jiafu Zhu, Zhicheng Dong, Xin-Jian He, Qianwen SunpEASY-T5-zero (Transgen, CT501). In vitro transcription was performed using T7 polymerase (Promega, P1300) with 0.3 mM ribonucleoside adenosine/cytosine/guanine triphosphate (rATP/rCTP/rGTP), 0.1 mM uridine

(8) Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus PathogenicityJournal of VirologyJuly 27, 2023Claudia Kristen-Burmann, Peter Rogger, Inês Berenguer Veiga, Stefanie Riebesehl, Julie Rappe, Nadine Ebert, Carmen A. Sautter, Jenna N. Kelly, Hanspeter Stalder, Rosina Ehmann, et al.the template for in vitro transcription (Promega RiboMAX Large Scale RNA Production Systems; cat. no. P1300) after linearization with ClaI (New England BioLabs). The RNA was purified by using a Macherey-Nagel

(9) Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variantsNature communicationsJuly 26, 2023de Melo GD, Perraud V, Alvarez F, Vieites-Prado A, Kim S, Kergoat L, Coleon A, Trüeb BS, Tichit M, Piazza A, et al.process and transcribed in vitro into RNA using the T7 RiboMaxTM Large Scale RNA Production System (P1300, Promega) and the m7G(5’)ppp(5’)G RNA Cap Structure Analog (#S1411, NEB) kits. Approximately 5 μg of

(10) Multicomponent Lipid Nanoparticles for RNA TransfectionPharmaceuticsApril 20, 2023Nataliya Gretskaya, Mikhail Akimov, Dmitry Andreev, Anton Zalygin, Ekaterina Belitskaya, Galina Zinchenko, Elena Fomina-Ageeva, Ilya Mikhalyov, Elena Vodovozova, Vladimir Bezuglovin vitro transcription reaction was performed using the RiboMAX™ Large Scale RNA Production System (P1300, Promega, Madison, WI, USA) using a modified nucleotide composition for capping and incorporating N1

(11) Investigating Environmental Matrices for Use in Avian Influenza Virus Surveillance—Surface Water, Sediments, and Avian Fecal SamplesMicrobiology SpectrumApril 13, 2023Ann Kathrin Ahrens, Hans-Christoph Selinka, Claudia Wylezich, Hubert Wonnemann, Ole Sindt, Hartmut H. Hellmer, Florian Pfaff, Dirk Höper, Thomas C. Mettenleiter, Martin Beer, et al.(no. 28306; Qiagen). For in vitro transcription, the RiboMAX Large Scale RNA Production System (no. P1300; Promega, Walldorf, Germany) was used. Priming was achieved with a T7 primer. After completion of the

(12) Deciphering the Genetic Basis of Silkworm Cocoon Colors Provides New Insights into Biological Coloration and Phenotypic DiversificationMolecular Biology and EvolutionFebruary 3, 2023Yaru Lu, Jiangwen Luo, Erxia An, Bo Lu, Yinqiu Wei, Xiang Chen, Kunpeng Lu, Shubo Liang, Hai Hu, Minjin Han, et al.homozygous screening. SgRNA was synthesized using T7 RiboMAX Express Large-Scale RNA Production System (P1300, Promega, USA) and then diluted to 1,000 ng/μl. Nine microliters of sgRNA were added to 1 μl of TrueCut Cas9 Protein v2

(13) ONE-seq: epitranscriptome and gene-specific profiling of NAD-capped RNANucleic Acids ResearchJanuary 25, 2023Kongyan Niu, Jinyang Zhang, Shuwen Ge, Dean Li, Kunfeng Sun, Yingnan You, Jiaqian Qiu, Kun Wang, Xueting Wang, Rui Liu, et al.in vitro transcription, 10 μM of double-stranded DNA (dsDNA) template in 100 μl transcription buffer (Promega, catalog: P1300), along with 1 mM of each of GTP, CTP and UTP, with 1mM ATP (for normal RNA), 4 mM NAD (for NAD-RNA)

(14) CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryosGenome BiologyJanuary 19, 2023Youkui Huang, Bao-Qing Gao, Quan Meng, Liang-Zhong Yang, Xu-Kai Ma, Hao Wu, Yu-Hang Pan, Li Yang, Dong Li, Ling-Ling Chenare listed in Additional file 2: Table S1. The IVTs were done with T7 polymerase (Promega, Cat. No. P1300) to produce gRNAs. After that, the gRNAs were purified from denatured PAGE gel. For mRNAs produced by

(15) Phosphorylation of enteroviral 2Apro at Ser/Thr125 benefits its proteolytic activity and viral pathogenesisJournal of Medical VirologyJanuary 1, 2023Yuya Wang, Wenjia Zou, Yan Niu, Sanyuan Wang, Bangtao Chen, Rui Xiong, Peng Zhang, Zhijun Luo, Yong Wu, Changfa Fan, et al.In vitro transcription was used to synthesize RNAs from the corresponding recombinant DNA templates (p1300; Promega). Protein was overexpressed according to the instructions of the RRL System (L4151; Promega). 2.11

(16) Cardiovirus leader proteins retarget RSK kinases toward alternative substrates to perturb nucleocytoplasmic trafficPLoS pathogensDecember 1, 2022Lizcano-Perret B, Lardinois C, Wavreil F, Hauchamps P, Herinckx G, Sorgeloos F, Vertommen D, Gatto L, Michiels T.pulser apparatus (Bio-Rad) with viral RNA produced by in vitro transcription (RiboMax transcription kit P1300, Promega). Supernatants were collected 48-72h after electroporation, when cytopathic effect was complete.

(17) PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunityCellAugust 18, 2022Wang J, Zhang X, Greene GH, Xu G, Dong X.#E1910 Wheat Germ Extract,Promega,Cat. #L4380 RiboMAX™ Large Scale RNA Production Systems,Promega,Cat. #P1300 Vaccinia Capping System,NEB,Cat. #M2080S Experimental Models: Organisms/Strains,Experimental Models:

(18) DCSr-NL: A Novel Method to Semiquantitatively Probe the Growth Rate of NacreACS OmegaJuly 12, 2022Li Yi, Bing Zou, Liping Xie, Rongqing Zhangreferred to the manufacturer’s instruction in the Large Scale RNA Production Systems–T7 (Promega, Code NO: P1300) kit to design double-stranded RNAs (dsRNAs). DsGFP, as a negative control, was designed by referring

(19) Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq)STAR ProtocolsJune 1, 2022Olivier Finet, Carlo Yague-Sanz, Damien HermandMicro Columns,Sigma-Aldrich,Cat#GE28-9034-08 RiboMAXTM Large Scale RNA Production Systems,Promega,Cat#P1300 RiboMinus Eukaryote Probe Mix v2,Ambion,Cat#A15017 RiboMinus Eukaryote Kit v2,Thermo Fisher Scientific,Cat#A15020 RiboMinus Concentration module

(20) Discovery and Characterization of an Aberrant Small Form of Glycoprotein I of Herpes Simplex Virus Type I in Cell CultureMicrobiology SpectrumApril 27, 2022Xixi Gui, Wuchao Zhang, Peng Gao, Yongning Zhang, Lei Zhou, Xinna Ge, Xin Guo, John W. Wills, Jun Han, Hanchun Yangto the protocol of the RiboMAX Large Scale RNA Production Systems—SP6 and T7 (Promega, catalog no. P1300). The in vitro transcribed gI mRNA was purified by Monarch RNA Cleanup Kit (New England Biolabs, catalog

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RiboMAX Large Scale RNA Production Systems (SP6 and T7) From Promega
I used this kit to produce RNA for injecting into Xenopus embryos. In order to transcribe RNA, your ... Read More